Qhov txawv ntawm zeatin, Trans-zeatin thiab zeatin riboside yog dab tsi? Lawv siv rau dab tsi?

Cov haujlwm tseem ceeb

1. Txhawb kev faib cell, feem ntau yog kev faib ntawm cytoplasm;

2. Txhawb kom muaj kev sib txawv ntawm cov noob. Hauv kev cog cov ntaub so ntswg, nws cuam tshuam nrog auxin los tswj kev sib txawv thiab kev tsim ntawm cov hauv paus hniav thiab cov noob;

3. Txhawb kev loj hlob ntawm cov paj lateral, tshem tawm cov apical dominance, thiab yog li ua rau muaj ntau cov paj adventitious hauv cov ntaub so ntswg;

4. Ncua cov nplooj ntoo laus, qeeb qeeb qhov kev puas tsuaj ntawm chlorophyll thiab cov protein;

5. Ua kom cov noob tsis loj hlob, hloov lub teeb kom tau raws li qhov xav tau ntawm cov noob xws li luam yeeb;

6. Ua rau parthenocarpy hauv qee cov txiv hmab txiv ntoo;

7. Txhawb kom muaj cov paj pib: ntawm qhov kawg ntawm nplooj thiab hauv qee cov mosses, nws tuaj yeem txhawb kom muaj cov paj pib;

8. Txhawb kom cov qos yaj ywm loj hlob zoo.

Nws tsuas muaj cov qauv trans thiab muaj cov nyhuv zoo ib yam lizeatin, tab sis nrog kev ua ub ua no muaj zog dua.

Nws cov nyhuv zoo ib yam li cov tshuaj tiv thaiv zeatin. Nws tsis yog tsuas yog muaj cov haujlwm ntawm zeatin saum toj no xwb, tab sis kuj muaj cov nyhuv ntawm kev ua kom cov noob caj noob ces thiab kev ua haujlwm ntawm cov metabolism.

Txoj Kev Siv

1. Txhawb kom cov callus germination (yuav tsum tau siv ua ke nrog auxin), concentration 1mg / L.

2. Txhawb kom cov txiv hmab txiv ntoo loj hlob, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, tsuag rau cov txiv hmab txiv ntoo 10, 25, thiab 40 hnub tom qab paj.

3. Rau cov zaub nplooj, tsuag ntawm 201 mg/L kom qeeb qhov nplooj daj.

Tsis tas li ntawd xwb, kev kho qee cov noob qoob loo tuaj yeem txhawb kev tawg paj; kev kho thaum lub sijhawm noob cog tuaj yeem txhawb kev loj hlob.

1. Txhawb kom cov ntaub so ntswg callus germination (yuav tsum siv ua ke nrog auxin), ntawm qhov concentration ntawm 1 ppm;

2. Txhawb kom cov txiv hmab txiv ntoo loj hlob, 100 ppm ntawm cytokinin + 500 ppm ntawm GA3 + 20 ppm ntawm NAA, tshuaj tsuag cov txiv hmab txiv ntoo 10, 25, thiab 40 hnub tom qab paj;

3. Ncua qhov daj ntawm nplooj zaub, tshuaj tsuag 20 ppm;

1. Hauv kev cog cov ntaub so ntswg, qhov feem ntau ntawm cov tshuaj anti-cytokinin nucleoside yog 1 mg / mL lossis siab dua.

2. Hauv kev tswj kev loj hlob ntawm cov nroj tsuag, qhov concentration ntawm anti-cytokinin nucleoside feem ntau yog 1 ppm txog 100 ppm, thiab qhov concentration tshwj xeeb nyob ntawm daim ntawv thov tshwj xeeb thiab hom nroj tsuag. Piv txwv li, thaum txhawb kev tawg ntawm cov ntaub so ntswg callus, qhov concentration ntawm anti-cytokinin nucleoside yog 1 ppm, thiab nws yuav tsum tau siv ua ke nrog auxin.

3. Yaj cov hmoov anti-cytokinin nucleoside kom huv si nrog 2-5 mL ntawm 1 M NaOH (lossis 1 M acetic acid lossis 1 M KOH), tom qab ntawd ntxiv dej ob zaug distilled lossis dej ultrapure los npaj cov tshuaj khaws cia ntawm 1 mg/mL lossis siab dua. Do thaum ntxiv dej kom ntseeg tau tias sib tov zoo. Cov tshuaj khaws cia yuav tsum tau aliquoted thiab khov cia kom tsis txhob rov khov-yaj dua. Dilute cov tshuaj khaws cia nrog cov kab lis kev cai kom txog thaum cov concentration xav tau, thiab npaj cov tshuaj ua haujlwm tam sim ntawd thiab siv nws tam sim ntawd.

Xaus lus, zeatin, abscisic acid thiab abscisic acid nucleotide txhua tus muaj lawv tus yam ntxwv hais txog cov qauv, kev ua haujlwm thiab kev siv ua haujlwm. Xaus lus, zeatin, abscisic acid thiab abscisic acid nucleotide txhua tus muaj lawv tus yam ntxwv hais txog cov qauv, kev ua haujlwm thiab kev siv ua haujlwm. Txawm li cas los xij, lawv txhua tus ua haujlwm ua cov tswj kev loj hlob ntawm cov nroj tsuag thiab ua lub luag haujlwm tseem ceeb hauv kev txhawb nqa kev loj hlob thiab kev txhim kho ntawm cov nroj tsuag.