Lub luag haujlwm tseem ceeb
1. Txhawb kev faib cell, feem ntau yog kev faib cov cytoplasm;
2. Txhawb nqa kev sib txawv ntawm lub paj. Hauv cov ntaub so ntswg kab lis kev cai, nws cuam tshuam nrog auxin los tswj qhov sib txawv thiab tsim ntawm cov hauv paus hniav thiab buds;
3. Txhawb txoj kev loj hlob ntawm lateral buds, tshem tawm apical dominance, thiab yog li ua rau tsim ib tug loj tus naj npawb ntawm adventitious buds nyob rau hauv cov ntaub so ntswg kab lis kev cai;
4. Ncua nplooj senescence, qeeb tus nqi degradation ntawm chlorophyll thiab cov proteins;
5. Tso cov noob dormancy, hloov lub teeb kom tau raws li qhov xav tau ntawm cov noob xws li haus luam yeeb;
6. Induce parthenocarpy nyob rau hauv tej txiv hmab txiv ntoo;
7. Txhawb kev tsim cov paj pib: ntawm qhov txiav xaus ntawm nplooj thiab hauv qee cov mosses, nws tuaj yeem txhawb kev tsim cov paj pib;
8. Txhawb kev tsim cov qos yaj ywm tubers.
Nws tsuas muaj cov qauv trans thiab muaj cov nyhuv tib yam lizeatin, tab sis nrog kev ua kom muaj zog.
Nws cov nyhuv zoo ib yam li cov tshuaj tiv thaiv zeatin. Nws tsis tsuas yog muaj cov haujlwm saum toj saud ntawm zeatin, tab sis kuj tseem muaj cov txiaj ntsig ntawm kev ua kom cov noob qhia thiab kev ua haujlwm metabolic.
Txoj kev siv
1. Txhawb kev germination ntawm callus (yuav tsum tau siv ua ke nrog auxin), concentration 1mg/L.
2. Txhawb nqa cov txiv hmab txiv ntoo, 1001 mg/L zeatin + 5001 mg/L GA3 + 201 mg/L NAA, tshuaj tsuag rau cov txiv hmab txiv ntoo 10, 25, thiab 40 hnub tom qab flowering.
3. Rau nplooj zaub, tshuaj tsuag ntawm 201 mg/L kom ncua nplooj daj.
Tsis tas li ntawd, kev kho qee cov noob qoob loo tuaj yeem txhawb kev loj hlob; kev kho mob ntawm cov yub theem tuaj yeem txhawb kev loj hlob.
1. Txhawb kev loj hlob ntawm cov ntaub so ntswg callus (yuav tsum tau siv ua ke nrog auxin), ntawm qhov concentration ntawm 1 ppm;
2. Txhawb nqa cov txiv hmab txiv ntoo, 100 ppm ntawm cytokinin + 500 ppm ntawm GA3 + 20 ppm ntawm NAA, tshuaj tsuag cov txiv hmab txiv ntoo 10, 25, thiab 40 hnub tom qab paj;
3. ncua lub yellowing ntawm zaub nplooj, tshuaj tsuag 20 ppm;
1. Nyob rau hauv cov nroj tsuag kab lis kev cai, cov concentration ntawm anti-cytokinin nucleoside yog 1 mg / mL los yog siab dua.
2. Hauv kev tswj kev loj hlob ntawm cov nroj tsuag, qhov concentration ntawm anti-cytokinin nucleoside feem ntau yog 1 ppm txog 100 ppm, thiab cov concentration tshwj xeeb nyob ntawm cov ntawv thov tshwj xeeb thiab cov nroj tsuag hom. Piv txwv li, thaum txhawb kev loj hlob ntawm callus cov ntaub so ntswg, qhov concentration ntawm anti-cytokinin nucleoside yog 1 ppm, thiab nws yuav tsum tau siv nrog auxin.
3. Dissolve lub anti-cytokinin nucleoside hmoov kom huv si nrog 2-5 mL ntawm 1 M NaOH (los yog 1 M acetic acid los yog 1 M KOH), ces ntxiv ob-distilled dej los yog ultrapure dej los npaj ib tug cia tov ntawm 1 mg / mL los yog siab dua concentration. Do thaum ntxiv dej kom paub meej sib tov. Cov tshuaj cia yuav tsum tau aliquoted thiab khov cia kom tsis txhob rov khov-thawing. Dilute cov tshuaj cia nrog cov kab lis kev cai nruab nrab mus rau qhov xav tau concentration, thiab npaj cov tshuaj ua haujlwm ntawm qhov chaw thiab siv tam sim ntawd.
Hauv kev xaus, zeatin, abscisic acid thiab abscisic acid nucleotide txhua tus muaj lawv tus kheej cov yam ntxwv ntawm cov qauv, kev ua haujlwm thiab kev ua haujlwm. Txawm li cas los xij, lawv txhua tus ua haujlwm ua cov tswj kev loj hlob ntawm cov nroj tsuag thiab ua lub luag haujlwm tseem ceeb hauv kev txhawb nqa kev loj hlob thiab kev loj hlob ntawm cov nroj tsuag.
Post lub sij hawm: Oct-22-2025