Uniconazoleyog triazolekev tswj kev loj hlob ntawm cov nroj tsuaguas yog siv dav los tswj qhov siab ntawm cov nroj tsuag thiab tiv thaiv cov noob cog loj hlob dhau. Txawm li cas los xij, lub tshuab molecular uas uniconazole inhibits seedling hypocotyl elongation tseem tsis meej, thiab tsuas muaj ob peb txoj kev tshawb fawb uas muab cov ntaub ntawv transcriptome thiab metabolome los tshawb nrhiav lub tshuab ntawm hypocotyl elongation. Ntawm no, peb tau pom tias uniconazole inhibits hypocotyl elongation hauv Suav paj zaub qhwv noob. Nthuav, raws li kev sib xyaw ua ke transcriptome thiab metabolome tsom xam, peb pom tias uniconazole cuam tshuam rau txoj kev "phenylpropanoid biosynthesis". Hauv txoj kev no, tsuas yog ib lub noob ntawm tsev neeg enzyme regulatory gene, BrPAL4, uas koom nrog lignin biosynthesis, tau downregulated heev. Tsis tas li ntawd, poov xab ib-hybrid thiab ob-hybrid assays tau qhia tias BrbZIP39 tuaj yeem khi ncaj qha rau thaj chaw txhawb nqa ntawm BrPAL4 thiab ua kom nws cov ntawv sau. Lub kaw lus kaw cov noob caj noob ces uas tus kab mob ua rau muaj qhov ua pov thawj ntxiv tias BrbZIP39 tuaj yeem tswj tau qhov hypocotyl elongation ntawm cov zaub qhwv Suav thiab hypocotyl lignin synthesis. Cov txiaj ntsig ntawm txoj kev tshawb fawb no muab cov kev nkag siab tshiab rau hauv cov txheej txheem tswj hwm molecular ntawm cloconazole hauv kev txwv tsis pub hypocotyl elongation ntawm cov zaub qhwv Suav. Nws tau lees paub thawj zaug tias cloconazole txo cov lignin los ntawm kev txwv tsis pub phenylpropanoid synthesis mediated los ntawm BrbZIP39-BrPAL4 module, yog li ua rau hypocotyl dwarfing hauv cov noob zaub qhwv Suav.
Zaub qhwv Suav (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) yog ib hom zaub qhwv Brassica thiab yog ib hom zaub qhwv txhua xyoo uas paub zoo thiab cog dav hauv kuv lub tebchaws (Wang et al., 2022; Yue et al., 2022). Nyob rau xyoo tas los no, qhov ntau thiab tsawg ntawm cov zaub qhwv Suav tau txuas ntxiv nthuav dav, thiab txoj kev cog qoob loo tau hloov pauv los ntawm kev cog noob ncaj qha mus rau kev cog noob thiab kev hloov pauv noob. Txawm li cas los xij, thaum cog noob thiab kev hloov pauv noob, kev loj hlob ntawm hypocotyl ntau dhau feem ntau ua rau cov noob ceg, ua rau cov noob tsis zoo. Yog li ntawd, kev tswj kev loj hlob ntawm hypocotyl ntau dhau yog qhov teeb meem tseem ceeb hauv kev cog noob thiab kev hloov pauv noob zaub qhwv Suav. Tam sim no, muaj ob peb txoj kev tshawb fawb uas koom ua ke cov ntaub ntawv transcriptomics thiab metabolomics los tshawb nrhiav cov txheej txheem ntawm hypocotyl elongation. Lub txheej txheem molecular uas chlorantazole tswj kev nthuav dav hypocotyl hauv zaub qhwv Suav tseem tsis tau kawm. Peb lub hom phiaj yog txheeb xyuas seb cov noob caj noob ces thiab cov txheej txheem molecular twg teb rau uniconazole-induced hypocotyl dwarfing hauv cov zaub qhwv Suav. Siv cov kev tshuaj xyuas transcriptome thiab metabolomic, nrog rau kev tshuaj xyuas yeast one-hybrid, dual luciferase assay, thiab virus-induced gene silencing (VIGS) assay, peb pom tias uniconazole tuaj yeem ua rau hypocotyl dwarfing hauv cov zaub qhwv Suav los ntawm kev txwv tsis pub lignin biosynthesis hauv cov noob zaub qhwv Suav. Peb cov txiaj ntsig muab cov kev nkag siab tshiab rau hauv cov txheej txheem tswj hwm molecular uas uniconazole inhibits hypocotyl elongation hauv cov zaub qhwv Suav los ntawm kev txwv tsis pub phenylpropanoid biosynthesis los ntawm BrbZIP39-BrPAL4 module. Cov txiaj ntsig no yuav muaj cov txiaj ntsig tseem ceeb rau kev txhim kho qhov zoo ntawm cov noob lag luam thiab pab txhawb kom ntseeg tau tias cov qoob loo thiab cov zaub zoo.
Lub BrbZIP39 ORF puv ntoob tau muab tso rau hauv pGreenll 62-SK los tsim cov effector, thiab daim BrPAL4 promoter fragment tau sib xyaw nrog pGreenll 0800 luciferase (LUC) reporter gene los tsim cov reporter gene. Cov effector thiab reporter gene vectors tau sib koom ua ke ua nplooj luam yeeb (Nicotiana benthamiana).
Yuav kom paub meej txog kev sib raug zoo ntawm cov metabolites thiab cov noob caj noob ces, peb tau ua qhov kev tshuaj xyuas metabolilome thiab transcriptome ua ke. Kev tshuaj xyuas txoj kev txhim kho KEGG qhia tau tias DEGs thiab DAMs tau sib koom ua ke hauv 33 txoj kev KEGG (Daim Duab 5A). Ntawm lawv, txoj kev "phenylpropanoid biosynthesis" yog qhov tseem ceeb tshaj plaws; txoj kev "photosynthetic carbon fixation", txoj kev "flavonoid biosynthesis", txoj kev "pentose-glucuronic acid interconversion", txoj kev "tryptophan metabolism", thiab txoj kev "starch-sucrose metabolism" kuj tseem ceeb heev. Daim ntawv qhia kub clustering (Daim Duab 5B) qhia tau tias DAMs cuam tshuam nrog DEGs tau muab faib ua ntau pawg, ntawm cov flavonoids yog pawg loj tshaj plaws, qhia tias txoj kev "phenylpropanoid biosynthesis" tau ua lub luag haujlwm tseem ceeb hauv hypocotyl dwarfism.
Cov kws sau ntawv tshaj tawm tias kev tshawb fawb tau ua tiav thaum tsis muaj kev sib raug zoo hauv kev lag luam lossis kev nyiaj txiag uas yuav raug txhais ua kev tsis sib haum xeeb.
Txhua lub tswv yim uas tau hais tawm hauv tsab xov xwm no tsuas yog cov tswv yim ntawm tus sau xwb thiab tsis tas yuav yog cov tswv yim ntawm cov koom haum koom tes, cov tshaj tawm, cov neeg kho, lossis cov neeg tshuaj xyuas. Txhua yam khoom uas tau soj ntsuam hauv tsab xov xwm no lossis cov lus thov uas lawv cov chaw tsim khoom ua tsis tau lees paub lossis pom zoo los ntawm tus tshaj tawm.
Lub sijhawm tshaj tawm: Lub Peb Hlis-24-2025