Uniconazoleyog triazolecog kev loj hlob regulatoruas yog dav siv los tswj cov nroj tsuag qhov siab thiab tiv thaiv seedling overgrowth. Txawm li cas los xij, cov txheej txheem molecular uas uniconazole inhibits yub hypocotyl elongation tseem tsis paub meej, thiab tsuas muaj qee qhov kev tshawb fawb uas muab cov ntaub ntawv transcriptome thiab metabolome los tshawb xyuas cov txheej txheem ntawm hypocotyl elongation. Ntawm no, peb pom tias uniconazole ho inhibited hypocotyl elongation nyob rau hauv Suav flowering cabbage seedlings. Interestingly, raws li kev sib koom ua ke transcriptome thiab metabolome tsom xam, peb pom tias uniconazole cuam tshuam rau "phenylpropanoid biosynthesis" txoj hauv kev. Nyob rau hauv txoj kev no, tsuas yog ib lub noob ntawm cov enzyme tswj cov noob tsev neeg, BrPAL4, uas koom nrog hauv lignin biosynthesis, tau txo qis. Tsis tas li ntawd, cov poov xab ib-hybrid thiab ob-hybrid kev soj ntsuam tau pom tias BrbZIP39 tuaj yeem khi ncaj qha rau thaj tsam txhawb nqa ntawm BrPAL4 thiab qhib nws cov ntawv sau. Tus kab mob-induced gene silencing system ntxiv ua pov thawj tias BrbZIP39 tuaj yeem tswj hwm hypocotyl elongation ntawm Suav cabbage thiab hypocotyl lignin synthesis. Cov txiaj ntsig ntawm txoj kev tshawb no muab kev nkag siab tshiab rau hauv cov txheej txheem tswj molecular ntawm cloconazole hauv inhibiting hypocotyl elongation ntawm Suav cabbage. Nws tau lees paub thawj zaug tias cloconazole txo cov ntsiab lus lignin los ntawm inhibiting phenylpropanoid synthesis kho los ntawm BrbZIP39-BrPAL4 module, yog li ua rau hypocotyl dwarfing hauv Suav cabbage seedlings.
Suav cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) belongs rau lub genus Brassica thiab yog ib tug paub zoo txhua xyoo cruciferous zaub dav zus nyob rau hauv kuv lub teb chaws (Wang li al., 2022; Yue et al., 2022). Nyob rau hauv xyoo tas los no, qhov ntau lawm ntawm Suav cauliflower tau txuas ntxiv nthuav dav, thiab txoj kev cog qoob loo tau hloov pauv los ntawm kev cog qoob loo ncaj qha mus rau cov noob qoob loo hnyav thiab hloov pauv. Txawm li cas los xij, nyob rau hauv tus txheej txheem ntawm intensive seedling kab lis kev cai thiab transplantation, ntau heev hypocotyl kev loj hlob nyhav tsim leggy seedlings, uas ua rau cov tsis zoo yub. Yog li ntawd, tswj kev loj hlob ntawm hypocotyl ntau dhau yog qhov teeb meem tseem ceeb hauv cov kab lis kev cai hnyav thiab kev hloov pauv ntawm Suav cabbage. Tam sim no, muaj ob peb txoj kev tshawb fawb sib koom ua ke transcriptomics thiab metabolomics cov ntaub ntawv los tshawb txog cov txheej txheem ntawm hypocotyl elongation. Lub tshuab molecular uas chlorantazole tswj kev nthuav dav hypocotyl hauv Suav cabbage tseem tsis tau kawm. Peb tsom los txheeb xyuas cov noob thiab cov txheej txheem molecular twg teb rau uniconazole-induced hypocotyl dwarfing hauv Suav cabbage. Siv transcriptome thiab metabolomic analyses, nrog rau cov poov xab ib-hybrid tsom xam, dual luciferase assay, thiab virus-induced gene silencing (VIGS) assay, peb pom tias uniconazole yuav induce hypocotyl dwarfing nyob rau hauv Suav cabbage los ntawm inhibiting lignin biosynthesis nyob rau hauv Suav cabbage seedlings. Peb cov txiaj ntsig tau muab kev nkag siab tshiab rau hauv cov txheej txheem tswj hwm molecular uas uniconazole inhibits hypocotyl elongation hauv Suav cabbage los ntawm inhibiting phenylpropanoid biosynthesis kho los ntawm BrbZIP39-BrPAL4 module. Cov txiaj ntsig no tuaj yeem muaj qhov cuam tshuam tseem ceeb rau kev txhim kho cov qoob loo lag luam zoo thiab ua kom muaj txiaj ntsig zoo ntawm cov qoob loo thiab cov zaub zoo.
Qhov puv-ntev BrbZIP39 ORF tau muab tso rau hauv pGreenll 62-SK los tsim cov nyhuv, thiab BrPAL4 tus txhawb nqa fragment tau fused rau pGreenll 0800 luciferase (LUC) reporter gene los tsim cov neeg sau xov xwm gene. Cov effector thiab reporter gene vectors tau co-transformed rau hauv luam yeeb (Nicotiana benthamiana) nplooj.
Txhawm rau kom paub meej txog kev sib raug zoo ntawm cov metabolites thiab cov noob, peb tau ua ib qho kev sib koom ua ke metabolome thiab transcriptome tsom xam. KEGG pathway enrichment analysis tau pom tias DEGs thiab DAMs tau sib koom ua ke hauv 33 KEGG txoj hauv kev (Daim duab 5A). Ntawm lawv, txoj kev "phenylpropanoid biosynthesis" yog qhov ua kom muaj txiaj ntsig zoo tshaj plaws; txoj kev "photosynthetic carbon fixation" txoj kev, "flavonoid biosynthesis" txoj kev, txoj kev "pentose-glucuronic acid interconversion" txoj kev, "tryptophan metabolism" txoj kev, thiab " starch-sucrose metabolism" txoj kev kuj tseem muaj txiaj ntsig zoo. Daim ntawv qhia tshav kub (Daim duab 5B) tau pom tias DAMs cuam tshuam nrog DEGs tau muab faib ua ntau pawg, ntawm cov flavonoids yog pawg loj tshaj plaws, qhia tias txoj hauv kev "phenylpropanoid biosynthesis" ua lub luag haujlwm tseem ceeb hauv hypocotyl dwarfism.
Cov kws sau ntawv tshaj tawm tias qhov kev tshawb fawb tau ua thaum tsis muaj kev lag luam lossis kev sib raug zoo nyiaj txiag uas tuaj yeem txhais tau tias muaj kev tsis sib haum xeeb ntawm kev txaus siab.
Txhua qhov kev xav uas tau hais hauv tsab xov xwm no tsuas yog cov neeg sau ntawv nkaus xwb thiab tsis tas yuav cuam tshuam txog kev xav ntawm cov koom haum koom nrog, cov tshaj tawm, cov neeg kho, lossis cov neeg tshuaj xyuas. Txhua yam khoom uas tau soj ntsuam hauv kab lus no lossis cov lus thov los ntawm lawv cov tuam txhab yuav tsis lav lossis pom zoo los ntawm tus tshaj tawm.
Post lub sij hawm: Mar-24-2025