Cov kws tshawb fawb los ntawm Department of Biochemistry ntawm Indian Institute of Sciences (IISc) tau tshawb pom lub tshuab tshawb nrhiav ntev.rau kev tswj kev loj hlob ntawm cov nroj tsuag hauv av thaum ub xws li bryophytes (ib pawg uas suav nrog mosses thiab liverworts) uas tau khaws cia hauv cov paj ntoo tom qab.
Txoj kev tshawb no, luam tawm nyob rau hauv phau ntawv journal Nature Chemical Biology, tsom rau cov kev cai uas tsis yog-canonical ntawm DELLA proteins, tus tswv txoj kev loj hlob regulator uas suppresses cell division nyob rau hauv embryophytes (cov nroj tsuag av).
Interestingly, bryophytes, thawj cov nroj tsuag tau tshwm sim hauv thaj av kwv yees li 500 lab xyoo dhau los, tsis muaj GID1 receptor, txawm tias tsim cov phytohormone GA. Qhov no ua rau cov lus nug txog yuav ua li cas kev loj hlob thiab kev loj hlob ntawm cov nroj tsuag thaum ntxov no tau tswj hwm.
Siv lub liverwort Marchantia polymorpha ua qauv qauv, cov kws tshawb fawb pom tias cov nroj tsuag thaum ub siv cov enzyme tshwj xeeb, MpVIH, uas tsim cov xov tooj ntawm tes inositol pyrophosphate (InsP₈), txhawm rau rhuav DELLA yam tsis muaj kev koom tes ntawmgibberellik acid.
Cov kws tshawb fawb tau siv CRISPR-Cas9 system los rhuav tshem cov noob encoding VIH enzyme, lees paub nws lub luag haujlwm. Cov nroj tsuag tsis muaj kev ua haujlwm VIH pom muaj kev loj hlob tsis xws luag thiab morphological abnormalities, xws li nplooj compact, impaired radial loj hlob, thiab tsis muaj calyxes. Cov teeb meem no tau raug tshem tawm los ntawm kev hloov kho cov genome cog kom tsim tau ib qho kawg (N-terminus) ntawm VIH enzyme. Siv cov txheej txheem chromatography siab heev, pab pawg tau tshawb pom tias N-terminus muaj kinase domain uas catalyzes zus tau tej cov InsP₈.
Cov kws tshawb nrhiav pom tias DELLA yog ib lub hom phiaj ntawm tes ntawm VIH kinase. Tsis tas li ntawd, lawv tau pom tias cov phenotypes ntawm MpVIH-tsis muaj cov nroj tsuag zoo ib yam li cov nroj tsuag M. polymorpha nrog nce DELLA qhia.
"Lub sijhawm no, peb zoo siab to taub tias DELLA kev ruaj ntseg lossis kev ua haujlwm tau txhim kho hauv MpVIH-tsis muaj cov nroj tsuag," said Priyanshi Rana, thawj tus kws sau ntawv thiab cov tub ntxhais kawm tiav hauv Lahey pawg tshawb fawb. Raws li lawv qhov kev xav, cov kws tshawb fawb pom tias DELLA inhibition tuaj yeem kho qhov kev loj hlob tsis zoo thiab kev loj hlob phenotypes ntawm MpVIH mutant nroj tsuag. Cov txiaj ntsig no qhia tias VIH kinase tsis zoo tswj DELLA, txhawb kev cog qoob loo thiab kev loj hlob.
Kev tshawb fawb rau DELLA cov proteins hnub rov qab mus rau Green Revolution, thaum cov kws tshawb fawb tsis paub siv lawv lub peev xwm los tsim cov khoom muaj txiaj ntsig zoo ib nrab-dwarf ntau yam. Txawm hais tias cov ntsiab lus ntawm lawv txoj haujlwm tsis meej nyob rau lub sijhawm, cov cuab yeej siv niaj hnub no tso cai rau cov kws tshawb fawb los tswj cov haujlwm ntawm cov proteins los ntawm kev tsim cov noob caj noob ces, ua kom cov qoob loo zoo.
Post lub sij hawm: Oct-23-2025