DELLA cov proteins tau khaws ciakev loj hlob regulatorsuas ua lub luag haujlwm tseem ceeb hauv kev tsim cov nroj tsuag hauv kev teb rau cov teeb liab sab hauv thiab sab nraud. Raws li cov tswj hwm kev hloov pauv, DELLAs khi rau cov khoom hloov pauv (TFs) thiab histone H2A ntawm lawv cov GRAS domains thiab raug xaiv los ua tus txhawb nqa. Cov kev tshawb fawb tsis ntev los no tau pom tias DELLA kev ruaj ntseg yog tswj hwm tom qab kev txhais lus los ntawm ob lub tswv yim: polyubiquitination induced los ntawm cov nroj tsuag hormone.gibberellin ua, uas ua rau lawv sai sai degradation, thiab conjugation nrog me me ubiquitin-zoo li hloov kho (SUMO), uas ua rau lawv tsub zuj zuj. Ntxiv mus, DELLA kev ua ub no yog tswj hwm los ntawm ob txoj kev sib txawv glycosylation: O-fucosylation txhim kho DELLA-TF kev sib cuam tshuam, thaum O-txuas N-acetylglucosamine (O-GlcNAc) hloov kho inhibits DELLA-TF kev sib cuam tshuam. Txawm li cas los xij, lub luag haujlwm ntawm DELLA phosphorylation tsis meej raws li cov kev tshawb fawb yav dhau los tau pom cov txiaj ntsig tsis sib haum xeeb, nrog qee qhov qhia tias phosphorylation txhawb nqa lossis ua rau DELLA degradation thiab lwm tus qhia tias phosphorylation tsis cuam tshuam rau lawv txoj kev ruaj ntseg. Ntawm no, peb txheeb xyuas qhov chaw phosphorylation nyob rau hauv GA1-3 repressor (RGA), AtDELLA, purified los ntawm Arabidopsis thaliana los ntawm huab hwm coj spectrometry thiab qhia tias phosphorylation ntawm ob RGA peptides nyob rau hauv lub PolyS thiab PolyS / T cheeb tsam txhim kho RGA kev ua si los ntawm kev txhawb H2A binding lub hom phiaj thiab RGA. Qhov tseem ceeb, phosphorylation tsis cuam tshuam rau RGA-TF kev sib cuam tshuam lossis RGA stability. Peb txoj kev tshawb fawb nthuav tawm cov txheej txheem molecular los ntawm phosphorylation induces DELLA kev ua haujlwm.
Peb qhov kev soj ntsuam loj spectrometric tau qhia tias Pep1 thiab Pep2 tau phosphorylated heev hauv RGA hauv GA-tsis muaj Ga1 keeb kwm yav dhau. Ntxiv rau qhov kev tshawb fawb no, kev tshawb fawb phosphoproteomic kuj tau qhia Pep1 phosphorylation hauv RGA, txawm hais tias nws lub luag haujlwm tseem tsis tau kawm txog 53,54,55. Hauv qhov sib piv, Pep2 phosphorylation tsis tau piav qhia yav dhau los txij li peptide no tsuas tuaj yeem kuaj pom siv RGAGKG transgene. Txawm hais tias m1A kev hloov pauv, uas tshem tawm Pep1 phosphorylation, tsuas yog txo qis RGA kev ua haujlwm hauv planta, nws muaj cov nyhuv ntxiv thaum ua ke nrog m2A hauv kev txo qis RGA kev ua haujlwm (Cov duab ntxiv 6). Qhov tseem ceeb, Pep1 phosphorylation tau txo qis hauv GA-enhanced sly1 mutant piv rau ga1, qhia tias GA txhawb nqa RGA dephosphorylation, txo nws txoj haujlwm. Lub tshuab uas GA suppresses RGA phosphorylation yuav tsum tau tshawb xyuas ntxiv. Ib qho ua tau yog tias qhov no tau ua tiav los ntawm kev tswj hwm ntawm cov protein kinase uas tsis paub meej. Txawm hais tias cov kev tshawb fawb tau pom tias kev qhia ntawm CK1 protein kinase EL1 yog downregulated los ntawm GA hauv mov 41, peb cov txiaj ntsig tau qhia tias kev hloov pauv siab dua ntawm Arabidopsis EL1 homologue (AEL1-4) tsis txo RGA phosphorylation. Raws li kev pom zoo nrog peb cov txiaj ntsig, kev tshawb fawb phosphoproteomic tsis ntev los no siv Arabidopsis AEL overexpressing kab thiab ael triple mutant tsis tau txheeb xyuas cov DELLA cov proteins uas yog cov substrates ntawm cov kinases56. Thaum peb npaj cov ntawv sau, nws tau tshaj tawm tias GSK3, cov noob encoding GSK3 / SHAGGY-zoo li kinase hauv cov nplej (Triticum aestivum), tuaj yeem phosphorylate DELLA (Rht-B1b)57, txawm tias phosphorylation ntawm Rht-B1b los ntawm GSK3 tsis tau lees paub hauv cov nroj tsuag. Nyob rau hauv vitro enzymatic cov tshuaj tiv thaiv nyob rau hauv lub xub ntiag ntawm GSK3 ua raws li huab hwm coj spectrometry tsom xam qhia peb phosphorylation qhov chaw nyob nruab nrab ntawm lub DELLA thiab GRAS domains ntawm Rht-B1b (Ntxiv daim duab 3). Serine rau alanine hloov pauv ntawm tag nrho peb qhov chaw phosphorylation ua rau txo qis Rht-B1b kev ua hauv cov nplej transgenic, raws li peb cov kev tshawb pom tias alanine hloov pauv hauv Pep2 RGA txo RGA kev ua haujlwm. Txawm li cas los xij, hauv vitro protein degradation kev soj ntsuam ntxiv tau pom tias phosphorylation tuaj yeem ua kom ruaj khov Rht-B1b57. Qhov no yog qhov sib piv rau peb cov txiaj ntsig uas qhia tias kev hloov pauv alanine hauv Pep2 RGA tsis hloov nws txoj kev ruaj ntseg hauv planta. GSK3 nyob rau hauv cov nplej yog ib qho ortholog ntawm brassinosteroid-insensitive protein 2 (BIN2) nyob rau hauv Arabidopsis 57. BIN2 yog ib tug tsis zoo regulator ntawm BR signaling, thiab BR activates nws signaling pathway los ntawm ua rau BIN2 degradation 58. Peb pom tau hais tias BR kev kho mob tsis txo RGA stabilityrabphomentary theem 59 los yog phosphorus. 2), qhia tias RGA tsis zoo li yuav phosphorylated los ntawm BIN2.
Tag nrho cov ntaub ntawv ntau tau raug txheeb xyuas los ntawm Excel, thiab qhov sib txawv tseem ceeb tau txiav txim siab siv Tub Ntxhais Kawm T-test. Tsis muaj cov txheej txheem txheeb cais tau siv los txiav txim siab ua ntej qhov loj me. Tsis muaj cov ntaub ntawv raug tshem tawm los ntawm kev txheeb xyuas; qhov kev sim tsis tau randomized; thiab cov kws tshawb fawb tau paub txog kev faib tawm thaum lub sij hawm sim thiab kev ntsuam xyuas cov txiaj ntsig. Cov qauv loj yog muab nyob rau hauv daim duab legends thiab hauv cov ntaub ntawv raw cov ntaub ntawv.
Lub sij hawm xa tuaj: Plaub Hlis-15-2025