DELLA proteins yog kev txuag tus tswvkev loj hlob regulatorsuas ua lub luag haujlwm tseem ceeb hauv kev tswj cov nroj tsuag kev loj hlob nyob rau hauv teb rau sab hauv thiab ib puag ncig cues. DELLA ua raws li kev tswj hwm kev hloov pauv thiab raug xaiv rau cov neeg txhawb nqa los ntawm kev khi rau cov khoom hloov pauv (TFs) thiab histone H2A los ntawm nws cov npe GRAS. Cov kev tshawb fawb tsis ntev los no tau pom tias DELLA kev ruaj ntseg yog tom qab kev txhais lus tswj los ntawm ob lub tswv yim: polyubiquitination induced los ntawm phytohormone gibberellin, uas ua rau nws sai degradation, thiab conjugation ntawm me me ubiquitin-zoo li modifiers (SUMO) kom nws tsub zuj zuj. Tsis tas li ntawd, DELLA kev ua haujlwm yog tswj hwm los ntawm ob qhov sib txawv glycosylations: DELLA-TF kev sib cuam tshuam yog txhim kho los ntawm O-fucosylation tab sis inhibited los ntawm O-txuas N-acetylglucosamine (O-GlcNAc) kev hloov kho. Txawm li cas los xij, lub luag haujlwm ntawm DELLA phosphorylation tseem tsis tau meej meej, raws li cov kev tshawb fawb yav dhau los tau pom cov txiaj ntsig tsis sib haum xeeb, xws li cov uas qhia tias phosphorylation txhawb lossis txo DELLA degradation rau lwm tus uas qhia tias phosphorylation tsis cuam tshuam rau nws txoj kev ruaj ntseg. Ntawm no, peb txheeb xyuas qhov chaw phosphorylation hauv REPRESSORib 1-3(RGA, AtDELLA) purified los ntawm Arabidopsis thaliana los ntawm huab hwm coj spectrometry tsom xam thiab qhia tau hais tias phosphorylation ntawm ob RGA peptides ntawm lub PolyS thiab PolyS / T cheeb tsam txhawb H2A khi thiab txhim kho RGA kev ua. Koom haum ntawm RGA nrog cov phiaj xwm txhawb nqa. Qhov tseem ceeb, phosphorylation tsis cuam tshuam rau RGA-TF kev sib cuam tshuam lossis RGA stability. Peb txoj kev tshawb fawb nthuav tawm cov txheej txheem molecular los ntawm phosphorylation induces DELLA kev ua haujlwm.
Txhawm rau kom paub meej lub luag haujlwm ntawm phosphorylation hauv kev tswj hwm DELLA muaj nuj nqi, nws yog ib qho tseem ceeb los txheeb xyuas qhov chaw DELLA phosphorylation hauv vivo thiab ua qhov kev soj ntsuam ua haujlwm hauv cov nroj tsuag. Los ntawm affinity purification ntawm cov nroj tsuag rho tawm raws li MS / MS tsom xam, peb txheeb xyuas ntau phosphosites hauv RGA. Nyob rau hauv tej yam kev mob ntawm GA deficiency, RHA phosphorylation nce, tab sis phosphorylation tsis cuam tshuam rau nws stability. Qhov tseem ceeb, co-IP thiab ChiP-qPCR kev soj ntsuam tau qhia tias phosphorylation ntawm PolyS / T cheeb tsam ntawm RGA txhawb nqa nws cov kev cuam tshuam nrog H2A thiab nws cov koom haum nrog cov phiaj xwm txhawb nqa, nthuav tawm cov txheej txheem uas phosphorylation induces RGA muaj nuj nqi.
RGA raug xaiv los tsom chromatin los ntawm kev sib cuam tshuam ntawm LHR1 subdomain nrog TF thiab tom qab ntawd khi rau H2A los ntawm nws cheeb tsam PolyS / T thiab PFYRE subdomain, tsim H2A-RGA-TF complex kom ruaj khov RGA. Phosphorylation ntawm Pep 2 nyob rau hauv cheeb tsam PolyS / T nruab nrab ntawm DELLA domain thiab GRAS domain los ntawm ib qho kinase unidentified txhim kho RGA-H2A khi. rgam2A mutant protein abolishes RGA phosphorylation thiab txais cov protein sib txawv los cuam tshuam nrog H2A khi. Qhov no ua rau muaj kev tsis txaus ntseeg ntawm kev sib cuam tshuam TF-rgam2A thiab kev sib cais ntawm rgam2A los ntawm lub hom phiaj chromatin. Daim duab no piav qhia tsuas yog RGA-mediated transcriptional repression. Ib qho piv txwv zoo sib xws tuaj yeem piav qhia rau RGA-mediated transcriptional activation, tshwj tsis yog tias H2A-RGA-TF complex yuav txhawb cov hom phiaj hloov pauv thiab dephosphorylation ntawm rgam2A yuav txo qis kev sau ntawv. Daim duab hloov los ntawm Huang et al.21.
Tag nrho cov ntaub ntawv ntau tau raug txheeb xyuas los ntawm Excel, thiab qhov sib txawv tseem ceeb tau txiav txim siab siv Student's t test. Tsis muaj cov txheej txheem txheeb cais tau siv los txiav txim siab ua ntej tus qauv loj. Tsis muaj cov ntaub ntawv raug tshem tawm los ntawm kev txheeb xyuas; qhov kev sim tsis tau randomized; cov kws tshawb fawb tsis tau dig muag rau kev faib cov ntaub ntawv thaum lub sij hawm sim thiab kev ntsuam xyuas ntawm cov txiaj ntsig. Tus qauv loj yog qhia nyob rau hauv daim duab lus dab neeg thiab cov ntaub ntawv qhov chaw.
Yog xav paub ntxiv txog kev tsim qauv, saib Natural Portfolio Report Abstract txuam nrog rau kab lus no.
Cov ntaub ntawv loj spectrometry proteomics tau pab txhawb rau ProteomeXchange consortium los ntawm PRIDE66 tus khub repository nrog dataset identifier PXD046004. Tag nrho lwm cov ntaub ntawv tau txais los ntawm txoj kev tshawb no tau nthuav tawm hauv Cov Ntaub Ntawv Ntxiv, Cov Ntaub Ntawv Ntxiv Ntxiv, thiab Cov Ntaub Ntawv Raw. Cov ntaub ntawv tau muab los ntawm kab lus no.
Post lub sij hawm: Nov-08-2024