Cov nceb loj muaj ntau yam khoom siv bioactive thiab suav tias yog cov khoom siv bioresources muaj nqis. Phellinus igniarius yog ib hom nceb loj uas ib txwm siv rau kev kho mob thiab kev noj haus, tab sis nws txoj kev faib tawm thiab lub npe Latin tseem muaj kev tsis sib haum xeeb. Los ntawm kev siv ntau hom noob caj noob ces sib txuas, cov kws tshawb fawb tau lees paub tias Phellinus igniarius thiab cov hom zoo sib xws yog cov genus tshiab thiab tau tsim cov genus Sanghuangporus. Cov nceb honeysuckle Sanghuangporus lonicericola yog ib hom Sanghuangporus uas tau txheeb xyuas thoob ntiaj teb. Phellinus igniarius tau nyiam mloog ntau vim nws cov khoom siv tshuaj ntau yam, suav nrog polysaccharides, polyphenols, terpenes, thiab flavonoids. Triterpenes yog cov tshuaj tseem ceeb ntawm cov genus no, uas ua rau muaj kev tiv thaiv antioxidant, antibacterial, thiab antitumor.
Triterpenoids muaj peev xwm loj heev rau kev siv ua lag luam. Vim yog qhov tsis tshua muaj cov peev txheej Sanghuangporus qus hauv xwm, kev txhim kho nws cov biosynthetic efficiency thiab cov txiaj ntsig yog qhov tseem ceeb heev. Tam sim no, kev vam meej tau ua tiav hauv kev txhim kho kev tsim ntau yam metabolites theem nrab ntawm Sanghuangporus los ntawm kev siv cov tshuaj inducers los tswj cov tswv yim fermentation hauv qab dej. Piv txwv li, polyunsaturated fatty acids, fungal elicitors11 thiab phytohormones (suav nrog methyl jasmonate thiab salicylic acid14) tau pom tias ua rau muaj triterpenoid ntau ntxiv hauv Sanghuangporus. Cov tshuaj tswj kev loj hlob ntawm cov nroj tsuag(PGRs)tuaj yeem tswj hwm qhov biosynthesis ntawm cov metabolites theem nrab hauv cov nroj tsuag. Hauv txoj kev tshawb fawb no, PBZ, tus tswj kev loj hlob ntawm cov nroj tsuag uas siv dav los tswj kev loj hlob ntawm cov nroj tsuag, cov qoob loo, qhov zoo thiab cov yam ntxwv ntawm lub cev, tau raug tshawb xyuas. Tshwj xeeb, kev siv PBZ tuaj yeem cuam tshuam rau txoj kev terpenoid biosynthetic hauv cov nroj tsuag. Kev sib xyaw ua ke ntawm gibberellins nrog PBZ ua rau cov ntsiab lus quinone methide triterpene (QT) ntau ntxiv hauv Montevidia floribunda. Cov khoom sib xyaw ntawm txoj kev terpenoid ntawm cov roj lavender tau hloov pauv tom qab kho nrog 400 ppm PBZ. Txawm li cas los xij, tsis muaj kev tshaj tawm txog kev siv PBZ rau cov nceb.
Ntxiv rau cov kev tshawb fawb uas tsom mus rau qhov nce ntxiv ntawm kev tsim cov triterpene, qee qhov kev tshawb fawb kuj tau piav qhia txog cov txheej txheem tswj hwm ntawm triterpene biosynthesis hauv Moriformis nyob rau hauv kev cuam tshuam ntawm cov tshuaj lom neeg inducers. Tam sim no, cov kev tshawb fawb tab tom tsom mus rau kev hloov pauv ntawm cov qib kev qhia tawm ntawm cov noob caj noob ces uas cuam tshuam nrog triterpene biosynthesis hauv MVA txoj kev, uas ua rau muaj kev nce ntxiv ntawm kev tsim cov terpenoid.12,14 Txawm li cas los xij, cov kev hauv qab cov noob caj noob ces paub no, tshwj xeeb tshaj yog cov yam ntxwv transcription tswj lawv qhov kev qhia tawm, tseem tsis meej hauv cov txheej txheem tswj hwm ntawm triterpene biosynthesis hauv Moriformis.
Hauv kev tshawb fawb no, cov teebmeem ntawm cov tshuaj tswj kev loj hlob ntawm cov nroj tsuag (PGRs) sib txawv ntawm kev tsim cov triterpene thiab kev loj hlob ntawm mycelial thaum lub sijhawm fermentation hauv qab dej ntawm honeysuckle (S. lonicericola) tau raug tshawb xyuas. Tom qab ntawd, cov metabolomics thiab transcriptomics tau siv los tshuaj xyuas cov khoom sib xyaw triterpene thiab cov qauv qhia txog cov noob caj noob ces uas koom nrog hauv triterpene biosynthesis thaum kho PBZ. RNA-sequencing thiab bioinformatics cov ntaub ntawv ntxiv tau txheeb xyuas cov hom phiaj transcription factor ntawm MYB (SlMYB). Ntxiv mus, cov mutants tau tsim los lees paub cov nyhuv tswj hwm ntawm SlMYB gene ntawm triterpene biosynthesis thiab txheeb xyuas cov noob caj noob ces uas muaj peev xwm. Electrophoretic mobility shift assays (EMSA) tau siv los lees paub kev sib cuam tshuam ntawm SlMYB protein nrog cov neeg txhawb nqa ntawm SlMYB target genes. Hauv kev xaus lus, lub hom phiaj ntawm txoj kev tshawb fawb no yog los txhawb kev siv triterpene biosynthesis siv PBZ thiab txheeb xyuas MYB transcription factor (SlMYB) uas tswj hwm cov noob triterpene biosynthetic suav nrog MVD, IDI, thiab FDPS hauv S. lonicericola los teb rau PBZ induction.
Kev ua kom ob qho tib si IAA thiab PBZ ua rau muaj ntau cov triterpenoid ntau ntxiv hauv honeysuckle, tab sis qhov cuam tshuam ntawm PBZ tau pom tseeb dua. Yog li ntawd, PBZ tau pom tias yog tus neeg ua kom zoo tshaj plaws ntawm qhov concentration ntxiv ntawm 100 mg / L, uas tsim nyog kawm ntxiv.
Lub sijhawm tshaj tawm: Lub Yim Hli-19-2025