Cov nceb loj muaj cov nplua nuj thiab ntau haiv neeg ntawm bioactive metabolites thiab suav tias yog bioresources tseem ceeb. Phellinus igniarius yog cov nceb loj ib txwm siv rau ob qho tib si tshuaj thiab zaub mov, tab sis nws qhov kev faib tawm thiab Latin lub npe tseem muaj teeb meem. Siv cov txheej txheem sib xyaw ua ke ntawm ntau hom, cov kws tshawb fawb tau lees paub tias Phellinus igniarius thiab cov tsiaj zoo sib xws yog cov genus tshiab thiab tsim cov genus Sanghuangporus. Lub honeysuckle nceb Sanghuangporus lonicericola yog ib hom kab mob Sanghuangporus thoob ntiaj teb. Phellinus igniarius tau txais txiaj ntsig zoo vim nws cov khoom siv tshuaj sib txawv, suav nrog polysaccharides, polyphenols, terpenes, thiab flavonoids. Triterpenes yog cov tshuaj tseem ceeb ntawm cov tshuaj nquag ntawm cov genus no, nthuav tawm cov tshuaj tua kab mob, tshuaj tua kab mob, thiab tshuaj tiv thaiv kab mob.
Triterpenoids muaj peev xwm zoo rau kev ua lag luam. Vim qhov tsis tshua muaj ntawm cov tsiaj qus Sanghuangporus hauv cov xwm txheej, kev txhim kho nws cov biosynthetic efficiency thiab tawm los yog qhov tseem ceeb heev. Tam sim no, kev nce qib tau ua tiav hauv kev txhim kho kev tsim tawm ntawm ntau yam metabolites theem nrab ntawm Sanghuangporus los ntawm kev siv tshuaj inducers los tswj cov tswv yim fermentation submerged. Piv txwv li, polyunsaturated fatty acids, fungal elicitors11 thiab phytohormones (xws li methyl jasmonate thiab salicylic acid14) tau pom tias nce triterpenoid ntau lawm hauv Sanghuangporus. Cog kev loj hlob regulators(PGRs)tuaj yeem tswj cov biosynthesis ntawm cov metabolites hauv cov nroj tsuag. Nyob rau hauv txoj kev tshawb no, PBZ, ib tug cog kev loj hlob regulator dav siv los tswj cov nroj tsuag kev loj hlob, tawm los, zoo thiab physiological zoo, raug soj ntsuam. Tshwj xeeb, kev siv PBZ tuaj yeem cuam tshuam txoj hauv kev terpenoid biosynthetic hauv cov nroj tsuag. Kev sib xyaw ntawm gibberellins nrog PBZ nce quinone methide triterpene (QT) cov ntsiab lus hauv Montevidia floribunda.Cov muaj pes tsawg leeg ntawm terpenoid txoj kev ntawm lavender roj tau hloov kho tom qab kho nrog 400 ppm PBZ. Txawm li cas los xij, tsis muaj lus ceeb toom txog kev siv PBZ rau nceb.
Ntxiv nrog rau cov kev tshawb fawb tsom mus rau kev nce hauv triterpene ntau lawm, qee qhov kev tshawb fawb kuj tau piav qhia txog cov txheej txheem tswj hwm ntawm triterpene biosynthesis hauv Moriformis nyob rau hauv kev cuam tshuam ntawm cov tshuaj inducers. Tam sim no, cov kev tshawb fawb tau tsom mus rau kev hloov pauv ntawm kev qhia theem ntawm cov txheej txheem genes cuam tshuam nrog triterpene biosynthesis hauv MVA txoj hauv kev, uas ua rau muaj kev nce hauv terpenoid ntau lawm.12,14 Txawm li cas los xij, cov txheej txheem hauv qab no paub txog cov noob caj noob ces, tshwj xeeb tshaj yog cov kev hloov pauv hauv kev tswj hwm lawv cov kev qhia, tseem tsis paub meej hauv cov txheej txheem kev tswj hwm kev tswj hwm.
Hauv txoj kev tshawb no, qhov cuam tshuam ntawm qhov sib txawv ntawm cov nroj tsuag kev loj hlob regulators (PGRs) ntawm triterpene ntau lawm thiab mycelial kev loj hlob thaum lub sij hawm submerged fermentation ntawm honeysuckle (S. lonicericola) raug soj ntsuam. Tom qab ntawd, metabolomics thiab transcriptomics tau siv los txheeb xyuas cov triterpene muaj pes tsawg leeg thiab cov qauv qhia cov noob koom nrog hauv triterpene biosynthesis thaum PBZ kho. RNA-sequencing thiab bioinformatics cov ntaub ntawv ntxiv txheeb xyuas lub hom phiaj kev hloov pauv ntawm MYB (SlMYB). Tsis tas li ntawd, cov mutants tau tsim los kom paub meej txog kev tswj xyuas cov txiaj ntsig ntawm SlMYB noob ntawm triterpene biosynthesis thiab txheeb xyuas cov hom phiaj muaj peev xwm. Electrophoretic mobility shift assays (EMSA) tau siv los txheeb xyuas qhov kev cuam tshuam ntawm SlMYB protein nrog cov neeg txhawb nqa ntawm SlMYB hom phiaj noob. Hauv cov ntsiab lus, lub hom phiaj ntawm txoj kev tshawb no yog los txhawb triterpene biosynthesis siv PBZ thiab txheeb xyuas MYB transcription factor (SlMYB) uas ncaj qha tswj triterpene biosynthetic noob suav nrog MVD, IDI, thiab FDPS hauv S. lonicericola hauv teb rau PBZ induction.
Induction ntawm ob qho tib si IAA thiab PBZ tau nce triterpenoid ntau lawm hauv honeysuckle, tab sis cov nyhuv induction ntawm PBZ tau tshaj tawm. Yog li, PBZ tau pom tias yog qhov zoo tshaj plaws inducer ntawm ib qho ntxiv ntawm 100 mg / L, uas tsim nyog kawm ntxiv.
Post lub sij hawm: Aug-19-2025