Nrog rau kev tsim tawm txhua xyoo ntawm ntau dua 700,000 tons, glyphosate yog cov tshuaj tua kab ntau tshaj plaws thiab siv ntau tshaj plaws hauv ntiaj teb. Cov nroj tsuag tsis kam thiab muaj peev xwm hem rau ecological ib puag ncig thiab tib neeg kev noj qab haus huv los ntawm kev tsim txom ntawm glyphosate tau nyiam heev.
Thaum Lub Tsib Hlis 29th, Xib Fwb Guo Ruiting pab pawg los ntawm Lub Xeev Lub Chaw Haujlwm Tseem Ceeb ntawm Biocatalysis thiab Enzyme Engineering, koom ua ke los ntawm Lub Tsev Kawm Ntawv ntawm Kev Tshawb Fawb Txog Lub Neej ntawm Hubei University thiab lub xeev thiab cov thawj coj saib xyuas, tau tshaj tawm cov ntawv tshawb fawb tshiab tshaj plaws hauv Phau ntawv Journal of Hazardous Materials, tshuaj xyuas. thawj tsom xam ntawm barnyard nyom. (Ib hom kab mob malignant paddy) - muab tau los ntawm aldo-keto reductase AKR4C16 thiab AKR4C17 catalyze cov tshuaj tiv thaiv cov txheej txheem ntawm glyphosate degradation, thiab zoo heev txhim kho cov degradation efficiency ntawm glyphosate los ntawm AKR4C17 los ntawm kev hloov molecular.
Loj hlob glyphosate kuj.
Txij li thaum nws cov lus qhia hauv xyoo 1970, glyphosate tau nrov thoob plaws ntiaj teb, thiab tau maj mam dhau los ua tus pheej yig tshaj plaws, feem ntau siv thiab ua tau zoo tshaj plaws broad-spectrum herbicide. Nws ua rau cov kab mob metabolic hauv cov nroj tsuag, suav nrog cov nroj tsuag, los ntawm tshwj xeeb inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), ib qho tseem ceeb enzyme koom nrog hauv cov nroj tsuag kev loj hlob thiab cov metabolism. thiab tuag.
Yog li ntawd, kev yug me nyuam glyphosate-resistant transgenic cov qoob loo thiab siv glyphosate hauv thaj teb yog ib txoj hauv kev tseem ceeb los tswj cov nroj tsuag hauv kev ua liaj ua teb niaj hnub no.
Txawm li cas los xij, nrog kev siv dav dav thiab kev tsim txom ntawm glyphosate, kaum ob ntawm cov nroj tsuag tau maj mam hloov zuj zus thiab txhim kho siab glyphosate kam rau ua.
Tsis tas li ntawd, glyphosate-resistant genetic hloov cov qoob loo tsis tuaj yeem decompose glyphosate, ua rau kev sib sau thiab hloov ntawm glyphosate hauv cov qoob loo, uas tuaj yeem kis tau yooj yim los ntawm cov khoom noj khoom haus thiab ua rau tib neeg kev noj qab haus huv.
Yog li ntawd, nws yog ib qho tseem ceeb kom nrhiav tau cov noob uas tuaj yeem degrade glyphosate, thiaj li cog qoob loo siab glyphosate-resistant transgenic cov qoob loo nrog tsawg glyphosate residues.
Kev daws cov qauv siv lead ua thiab cov tshuaj tiv thaiv catalytic ntawm cov nroj tsuag-derived glyphosate-degrading enzymes
Hauv xyoo 2019, pawg tshawb fawb Suav thiab Australian tau txheeb xyuas ob lub glyphosate-degrading aldo-keto reductases, AKR4C16 thiab AKR4C17, thawj zaug los ntawm glyphosate-resistant barnyard nyom. Lawv tuaj yeem siv NADP + ua cofactor kom degrade glyphosate rau nontoxic aminomethylphosphonic acid thiab glyoxylic acid.
AKR4C16 thiab AKR4C17 yog thawj zaug tshaj tawm glyphosate-degrading enzymes tsim los ntawm ntuj evolution ntawm cov nroj tsuag. Txhawm rau txhawm rau tshawb nrhiav ntxiv cov txheej txheem molecular ntawm lawv cov degradation ntawm glyphosate, Guo Ruiting pab neeg siv X-ray crystallography los tshuaj xyuas kev sib raug zoo ntawm ob enzymes thiab cofactor siab. Cov txheej txheem nyuaj ntawm kev daws teeb meem tau nthuav tawm txoj kev khi ntawm ternary complex ntawm glyphosate, NADP + thiab AKR4C17, thiab tau npaj cov tshuaj tiv thaiv catalytic ntawm AKR4C16 thiab AKR4C17-mediated glyphosate degradation.
Cov qauv ntawm AKR4C17 / NADP + / glyphosate complex thiab cov tshuaj tiv thaiv mechanism ntawm glyphosate degradation.
Molecular hloov kho txhim kho qhov degradation efficiency ntawm glyphosate.
Tom qab tau txais cov qauv zoo peb-dimensional ntawm AKR4C17 / NADP + / glyphosate, xibfwb Guo Ruiting pab pawg ntxiv tau txais cov protein mutant AKR4C17F291D nrog 70% nce hauv kev ua haujlwm degradation ntawm glyphosate los ntawm kev txheeb xyuas cov qauv enzyme thiab kev tsim qauv tsim nyog.
Kev soj ntsuam ntawm glyphosate-degrading kev ua ntawm AKR4C17 mutants.
"Peb txoj haujlwm nthuav tawm cov txheej txheem molecular ntawm AKR4C16 thiab AKR4C17 catalyzing degradation ntawm glyphosate, uas nteg lub hauv paus tseem ceeb rau kev hloov kho ntxiv ntawm AKR4C16 thiab AKR4C17 los txhim kho lawv cov degradation efficiency ntawm glyphosate." Raws li tus kws sau ntawv ntawm daim ntawv, Associate Professor Dai Longhai ntawm Hubei University tau hais tias lawv tsim cov protein mutant AKR4C17F291D nrog kev txhim kho glyphosate degradation efficiency, uas muab cov cuab yeej tseem ceeb rau kev cog qoob loo siab glyphosate-resistant transgenic cov qoob loo nrog tsawg glyphosate residues thiab siv cov kab mob microbial engineering. degrade glyphosate hauv ib puag ncig.
Nws tau tshaj tawm tias Guo Ruiting cov pab pawg tau koom nrog kev tshawb fawb ntev ntawm cov qauv kev tshuaj xyuas thiab kev sib tham txog kev sib tham ntawm biodegradation enzymes, terpenoid synthases, thiab tshuaj yeeb tshuaj cov proteins ntawm cov tshuaj lom thiab teeb meem hauv ib puag ncig. Li Hao, tus kws tshawb fawb koom nrog Yang Yu thiab tus kws qhia Hu Yumei hauv pab pawg yog thawj tus kws sau ntawv, thiab Guo Ruiting thiab Dai Longhai yog cov kws sau ntawv sib tham.
Post lub sij hawm: Jun-02-2022