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Exogenous gibberellic acid thiab benzylamine modulate txoj kev loj hlob thiab chemistry ntawm Schefflera dwarfis: ib tug stepwise regression tsom xam

Ua tsaug rau koj tuaj xyuas Nature.com.Cov version ntawm browser koj siv tau txwv CSS kev txhawb nqa.Txhawm rau kom tau txais txiaj ntsig zoo tshaj plaws, peb xav kom koj siv qhov tshiab ntawm koj tus browser (lossis lov tes taw Compatibility Hom hauv Internet Explorer).Lub sijhawm no, txhawm rau kom muaj kev txhawb nqa tsis tu ncua, peb tab tom qhia lub xaib tsis muaj styling lossis JavaScript.
Kho kom zoo nkauj foliage nroj tsuag nrog ib tug lush tsos yog heev muaj nuj nqis.Ib txoj hauv kev kom ua tiav qhov no yog sivcog kev loj hlob regulatorsraws li cov cuab yeej tswj kev cog qoob loo.Txoj kev tshawb no tau ua los ntawm Schefflera dwarf (ib tsob ntoo ornamental foliage) kho nrog foliar txau ntawmgibberellik acidthiab benzyladenine hormone nyob rau hauv ib lub tsev cog khoom nruab nrog ib tug pos huab irrigation system.Cov tshuaj no tau muab txau rau ntawm nplooj ntawm dwarf schefflera ntawm qhov ntau ntawm 0, 100 thiab 200 mg / l hauv peb theem txhua 15 hnub.Qhov kev sim tau ua nyob rau hauv lub hauv paus factorial nyob rau hauv ib tug kiag li randomized tsim nrog plaub replications.Kev sib xyaw ntawm gibberellic acid thiab benzyladenine ntawm qhov concentration ntawm 200 mg / l muaj qhov cuam tshuam loj rau cov nplooj, nplooj nplooj thiab qhov siab ntawm tsob ntoo.Qhov kev kho no kuj ua rau cov ntsiab lus siab tshaj plaws ntawm photosynthetic pigments.Tsis tas li ntawd, qhov sib piv siab tshaj plaws ntawm cov carbohydrates yooj yim thiab txo cov suab thaj tau pom nrog benzyladenine ntawm 100 thiab 200 mg / L thiab gibberellic acid + benzyladenine ntawm 200 mg / L.Stepwise regression tsom xam pom tias lub hauv paus ntim yog thawj qhov sib txawv ntawm tus qauv, piav qhia txog 44% ntawm qhov sib txawv.Qhov sib txawv tom ntej no yog cov hauv paus loj, nrog rau tus qauv bivariate piav qhia 63% ntawm qhov sib txawv ntawm nplooj ntawv.Qhov txiaj ntsig zoo tshaj plaws ntawm cov nplooj ntoo tau raug tshem tawm los ntawm cov hauv paus tshiab hnyav (0.43), uas yog qhov zoo sib xws nrog nplooj naj npawb (0.47).Cov txiaj ntsig tau pom tias gibberellic acid thiab benzyladenine ntawm qhov concentration ntawm 200 mg / l tau txhim kho kev loj hlob ntawm morphological, chlorophyll thiab carotenoid synthesis ntawm Liriodendron tulipifera, thiab txo cov ntsiab lus ntawm cov suab thaj thiab soluble carbohydrates.
Schefflera arborescens (Hayata) Merr yog ib tsob nroj ntsuab ntsuab ntawm tsev neeg Araliaceae, ib txwm nyob rau Suav teb thiab Taiwan1.Cov nroj tsuag no feem ntau loj hlob raws li houseplants, tab sis tsuas yog ib tsob nroj tuaj yeem loj hlob hauv cov xwm txheej zoo li no.Cov nplooj muaj los ntawm 5 mus rau 16 nplooj ntawv, txhua 10-20 cm2 ntev.Dwarf Schefflera tau muag ntau txhua xyoo, tab sis niaj hnub kev ua liaj ua teb tsis tshua siv.Yog li ntawd, kev siv cov nroj tsuag kev loj hlob regulators raws li zoo tswj cov cuab yeej los txhim kho kev loj hlob thiab sustainable zus tau tej cov horticultural khoom yuav tsum tau ntau yam kev saib xyuas.Niaj hnub no, kev siv cov nroj tsuag kev loj hlob regulators tau nce 3,4,5.Gibberellic acid yog cov tshuaj tswj kev loj hlob ntawm cov nroj tsuag uas tuaj yeem ua rau cov qoob loo nce ntxiv 6.Ib qho ntawm nws cov txiaj ntsig paub yog qhov kev txhawb nqa ntawm kev loj hlob, suav nrog qia thiab hauv paus elongation thiab nce nplooj nplooj7.Qhov tseem ceeb tshaj plaws ntawm gibberellins yog qhov nce ntawm qia qhov siab vim qhov ntev ntawm internodes.Foliar txau ntawm gibberellins ntawm cov nroj tsuag dwarf uas tsis tuaj yeem tsim cov gibberellins ua rau cov qia elongation thiab cog qhov siab 8.Foliar txau ntawm paj thiab nplooj nrog gibberellic acid ntawm ib tug concentration ntawm 500 mg / l tuaj yeem ua rau cov nroj tsuag qhov siab, tus naj npawb, qhov dav thiab ntev ntawm nplooj 9.Gibberellins tau raug tshaj tawm los txhawb kev loj hlob ntawm ntau yam broadleaf nroj tsuag10.Cov qia elongation tau pom hauv Scots ntoo thuv (Pinussylvestris) thiab dawb spruce (Piceaglauca) thaum nplooj tau txau nrog gibberellic acid11.
Ib txoj kev tshawb fawb tau tshuaj xyuas cov teebmeem ntawm peb cytokinin cog kev loj hlob regulators ntawm lateral ceg tsim nyob rau hauv Lily officinalis.khoov Kev sim tau ua nyob rau lub caij nplooj zeeg thiab caij nplooj ntoos hlav los kawm txog kev cuam tshuam rau lub caij nyoog.Cov txiaj ntsig tau pom tias kinetin, benzyladenine thiab 2-prenyladenine tsis cuam tshuam rau kev tsim cov ceg ntxiv.Txawm li cas los xij, 500 ppm benzyladenine ua rau muaj kev tsim ntawm 12.2 thiab 8.2 cov ceg ntoo hauv lub caij nplooj zeeg thiab caij nplooj ntoos hlav thwmsim, feem, piv rau 4.9 thiab 3.9 ceg hauv cov nroj tsuag tswj.Cov kev tshawb fawb tau pom tias kev kho lub caij ntuj sov muaj txiaj ntsig zoo dua li lub caij ntuj no12.Hauv lwm qhov kev sim, Kev Thaj Yeeb Lily var.Tassone nroj tsuag tau kho nrog 0, 250 thiab 500 ppm benzyladenine hauv 10 cm txoj kab uas hla.Cov txiaj ntsig tau pom tias cov av kho tau nce ntau cov nplooj ntxiv piv rau kev tswj hwm thiab cov nroj tsuag kho benzyladenine.Cov nplooj tshiab tshiab tau pom plaub lub lis piam tom qab kev kho mob, thiab cov nplooj ntau tshaj plaws tau pom tau yim lub lis piam tom qab kho.Thaum 20 lub lis piam tom qab kev kho mob, cov nroj tsuag kho av muaj qhov siab tsawg dua li cov nroj tsuag uas tau kho ua ntej13.Nws tau raug tshaj tawm tias benzyladenine ntawm qhov concentration ntawm 20 mg / L tuaj yeem ua rau cov nroj tsuag qhov siab thiab cov nplooj muaj pes tsawg leeg hauv Croton 14. Hauv calla lilies, benzyladenine ntawm qhov concentration ntawm 500 ppm ua rau muaj kev nce ntawm cov ceg ntoo, thaum tus naj npawb. ntawm ceg yog qhov tsawg kawg nkaus hauv pawg tswj hwm 15.Lub hom phiaj ntawm txoj kev tshawb no yog los tshawb xyuas foliar txau ntawm gibberellic acid thiab benzyladenine los txhim kho kev loj hlob ntawm Schefflera dwarfa, ib tsob nroj ornamental.Cov kws tswj kev loj hlob ntawm cov nroj tsuag no tuaj yeem pab cov neeg cog qoob loo ua lag luam npaj tsim khoom tsim nyog txhua xyoo.Tsis muaj kev tshawb fawb tau ua los txhim kho kev loj hlob ntawm Liriodendron tulipifera.
Txoj kev tshawb no tau ua nyob rau hauv tsev cog qoob loo tshawb fawb tsev cog khoom ntawm Islamic Azad University hauv Jiloft, Iran.Uniform root transplants ntawm dwarf schefflera 25 ± 5 cm nyob rau hauv qhov siab tau npaj (propagated rau lub hlis ua ntej kev sim) thiab sown nyob rau hauv pots.Lub lauj kaub yog yas, dub, nrog txoj kab uas hla ntawm 20 cm thiab qhov siab ntawm 30 cm16.
Cov kab lis kev cai nruab nrab hauv txoj kev tshawb no yog sib xyaw ntawm peat, humus, ntxuav xuab zeb thiab mov husk hauv qhov sib piv ntawm 1: 1: 1: 1 (los ntawm ntim) 16.Muab ib txheej ntawm pebbles rau hauv qab ntawm lub lauj kaub rau cov dej ntws.Nruab nrab nruab hnub thiab hmo ntuj kub nyob rau hauv lub tsev xog paj nyob rau hauv lig caij nplooj ntoos hlav thiab lub caij ntuj sov yog 32 ± 2 ° C thiab 28 ± 2 ° C, feem.Relative humidity yog> 70%.Siv ib lub tshuab mting rau irrigation.Qhov nruab nrab, cov nroj tsuag yog watered 12 zaug ib hnub twg.Nyob rau lub caij nplooj zeeg thiab lub caij ntuj sov, lub sij hawm ntawm txhua watering yog 8 feeb, lub sij hawm watering yog 1 teev.Cov nroj tsuag zoo sib xws tau loj hlob plaub zaug, 2, 4, 6 thiab 8 lub lis piam tom qab sowing, nrog rau cov tshuaj micronutrient (Ghoncheh Co., Iran) ntawm ib qho concentration ntawm 3 ppm thiab irrigated nrog 100 ml ntawm kev daws txhua zaus.Cov tshuaj muaj pes tsawg leeg muaj N 8 ppm, P 4 ppm, K 5 ppm thiab kab kawm Fe, Pb, Zn, Mn, Mo thiab B.
Peb qhov concentrations ntawm gibberellic acid thiab cov nroj tsuag kev loj hlob regulator benzyladenine (yuav los ntawm Sigma) tau npaj ntawm 0, 100 thiab 200 mg / L thiab txau rau cov nroj tsuag hauv peb theem ntawm ib ntus ntawm 15 hnub 17.Tween 20 (0.1%) (yuav los ntawm Sigma) tau siv rau hauv kev daws teeb meem kom nws lub neej ntev thiab kev nqus tau.Thaum sawv ntxov, tshuaj tsuag cov tshuaj hormones rau ntawm nplooj thiab nplooj ntawm Liriodendron tulipifera siv lub sprayer.Cov nroj tsuag yog txau nrog dej distilled.
Cog qhov siab, qia kab, nplooj cheeb tsam, chlorophyll cov ntsiab lus, tus naj npawb ntawm internodes, qhov ntev ntawm ob ceg, tus naj npawb ntawm cov ceg ob, cov hauv paus ntim, cov hauv paus hniav ntev, qhov loj ntawm nplooj, cag, qia thiab qhuav tshiab teeb meem, cov ntsiab lus ntawm photosynthetic pigments (chlorophyll a, chlorophyll b) Tag nrho chlorophyll, carotenoids, tag nrho cov pigments), txo cov suab thaj thiab soluble carbohydrates tau ntsuas hauv kev kho mob sib txawv.
Cov ntsiab lus chlorophyll ntawm cov nplooj hluas raug ntsuas 180 hnub tom qab txau siv cov tshuaj chlorophyll (Spad CL-01) thaum 9:30 txog 10 teev sawv ntxov (vim nplooj tshiab).Tsis tas li ntawd, thaj chaw nplooj tau ntsuas 180 hnub tom qab txau.Ntsuas peb nplooj ntawm sab saum toj, nruab nrab thiab hauv qab ntawm qia los ntawm txhua lub lauj kaub.Cov nplooj no yog siv los ua cov qauv ntawm daim ntawv A4 thiab cov qauv tshwm sim raug txiav tawm.Qhov hnyav thiab qhov chaw ntawm ib daim ntawv ntawm A4 daim ntawv kuj tau ntsuas.Tom qab ntawd thaj tsam ntawm cov nplooj stenciled yog suav nrog cov proportions.Tsis tas li ntawd, qhov ntim ntawm cov hauv paus hniav tau txiav txim siab siv lub tog raj kheej tiav.Nplooj qhuav qhov hnyav, qia qhuav qhov hnyav, cag qhuav qhov hnyav, thiab tag nrho qhov hnyav ntawm txhua tus qauv raug ntsuas los ntawm qhov cub kom qhuav ntawm 72 ° C rau 48 teev.
Cov ntsiab lus ntawm chlorophyll thiab carotenoids tau ntsuas los ntawm Lichtenthaler method18.Txhawm rau ua qhov no, 0.1 g ntawm nplooj tshiab tau muab tso rau hauv ib lub porcelain mortar uas muaj 15 ml ntawm 80% acetone, thiab tom qab lim, lawv qhov ntom ntom tau ntsuas siv lub spectrophotometer ntawm wavelengths ntawm 663.2, 646.8 thiab 470 nm.Calibrate lub cuab yeej siv 80% acetone.Xam cov concentration ntawm photosynthetic pigments siv cov kab zauv hauv qab no:
Ntawm lawv, Chl a, Chl b, Chl T thiab Tsheb sawv cev rau chlorophyll a, chlorophyll b, tag nrho chlorophyll thiab carotenoids, feem.Cov txiaj ntsig tau nthuav tawm hauv cov nroj tsuag mg / ml.
Kev txo cov suab thaj tau ntsuas siv txoj kev Somogy19.Ua li no, 0.02 g ntawm cov nroj tsuag tua yog hauv av nyob rau hauv ib tug porcelain mortar nrog 10 ml ntawm distilled dej thiab ncuav rau hauv ib lub khob me me.Kub lub khob kom npau npau thiab lim cov ntsiab lus siv Whatman No. 1 lim ntawv kom tau txais cov nroj tsuag extract.Hloov 2 ml ntawm txhua cov extract rau hauv lub raj kuaj thiab ntxiv 2 ml ntawm tooj liab sulfate tov.Npog lub raj kuaj nrog paj rwb ntaub plaub thiab kub hauv dej da dej ntawm 100 ° C rau 20 feeb.Nyob rau theem no, Cu2 + hloov mus rau Cu2O los ntawm kev txo cov aldehyde monosaccharide thiab cov xim salmon (terracotta) pom nyob hauv qab ntawm lub raj kuaj.Tom qab lub raj ntsuas tau txias, ntxiv 2 ml ntawm phosphomolybdic acid thiab xim xiav yuav tshwm.Co lub raj siv zog kom txog thaum cov xim sib npaug sib npaug thoob plaws hauv lub raj.Nyeem qhov nqus ntawm cov tshuaj ntawm 600 nm siv lub spectrophotometer.
Xam qhov concentration ntawm kev txo cov suab thaj siv tus qauv nkhaus.Qhov concentration ntawm soluble carbohydrates tau txiav txim los ntawm Fales txoj kev 20.Txhawm rau ua qhov no, 0.1 g ntawm sprouts tau tov nrog 2.5 ml ntawm 80% ethanol ntawm 90 ° C rau 60 min (ob theem ntawm 30 min txhua) kom tshem tawm cov carbohydrates yooj yim.Tom qab ntawd cov extract yog lim thiab cawv yog evaporated.Lub resulting precipitate yog yaj nyob rau hauv 2.5 ml ntawm distilled dej.Ncuav 200 ml ntawm txhua tus qauv rau hauv lub raj kuaj thiab ntxiv 5 ml ntawm anthrone qhia.Qhov sib tov tau muab tso rau hauv dej da dej ntawm 90 ° C rau 17 min, thiab tom qab txias, nws qhov nqus tau txiav txim siab ntawm 625 nm.
Qhov kev sim yog ib qho kev sim factorial raws li tus qauv tsim ua tiav nrog plaub replications.Cov txheej txheem PROC UNIVARIATE yog siv los tshuaj xyuas qhov qub ntawm cov ntaub ntawv xa tawm ua ntej kev txheeb xyuas qhov sib txawv.Kev txheeb xyuas kev txheeb xyuas pib nrog kev piav qhia txog kev txheeb xyuas kom nkag siab txog qhov zoo ntawm cov ntaub ntawv nyoos sau.Kev suav yog tsim los ua kom yooj yim thiab nthuav cov ntaub ntawv loj los ua kom lawv txhais tau yooj yim.Tom qab ntawd, kev tshuaj xyuas ntau dua tau ua tiav.Duncan qhov kev sim tau ua tiav siv SPSS software (version 24; IBM Corporation, Armonk, NY, USA) los xam cov squares thiab kev sim ua yuam kev los txiav txim qhov sib txawv ntawm cov ntaub ntawv teev.Duncan's ntau qhov kev xeem (DMRT) tau siv los txheeb xyuas qhov sib txawv ntawm cov txhais tau tias ntawm qhov tseem ceeb ntawm (0.05 ≤ p).Pearson correlation coefficient (r) tau suav nrog SPSS software (version 26; IBM Corp., Armonk, NY, USA) los ntsuas qhov sib txheeb ntawm cov khub sib txawv.Tsis tas li ntawd, kev tshuaj xyuas kab rov tav tau ua tiav siv SPSS software (v.26) los kwv yees qhov txiaj ntsig ntawm thawj xyoo hloov pauv raws li qhov muaj txiaj ntsig ntawm qhov hloov pauv xyoo thib ob.Ntawm qhov tod tes, kev ntsuam xyuas kev rov qab mus rau theem nrog p <0.01 tau ua los txheeb xyuas cov yam ntxwv uas cuam tshuam rau dwarf schefflera nplooj.Txoj kev tsom xam tau ua los txiav txim siab qhov cuam tshuam ncaj qha thiab tsis ncaj ntawm txhua tus cwj pwm hauv tus qauv (raws li cov yam ntxwv uas zoo dua piav qhia qhov txawv).Tag nrho cov kev suav sau saum toj no (ib txwm ntawm kev faib cov ntaub ntawv, kev sib raug zoo coefficient yooj yim, kev nce qib thiab kev tsom xam) tau ua los ntawm kev siv SPSS V.26 software.
Cov qauv cog qoob loo raug xaiv tau ua raws li cov koom haum cuam tshuam, lub teb chaws thiab thoob ntiaj teb cov lus qhia thiab kev cai lij choj hauv tebchaws Iran.
Table 1 qhia piav qhia cov txheeb cais ntawm qhov nruab nrab, tus qauv sib txawv, qhov tsawg kawg nkaus, siab tshaj plaws, ntau yam, thiab phenotypic coefficient ntawm variation (CV) rau ntau yam zoo.Ntawm cov txheeb cais no, CV tso cai rau kev sib piv ntawm cov cwj pwm vim nws tsis muaj qhov dav.Txo cov suab thaj (40.39%), hauv paus qhuav qhov hnyav (37.32%), hauv paus tshiab qhov hnyav (37.30%), qab zib rau qab zib piv (30.20%) thiab hauv paus ntim (30%) yog siab tshaj.thiab cov ntsiab lus chlorophyll (9.88%).) thiab nplooj nplooj muaj qhov ntsuas siab tshaj plaws (11.77%) thiab muaj tus nqi qis tshaj CV.Table 1 qhia tau hais tias tag nrho qhov hnyav ntub dej muaj qhov siab tshaj plaws.Txawm li cas los xij, qhov kev zoo no tsis muaj qhov siab tshaj CV.Yog li ntawd, qhov ntsuas tsis muaj qhov ntsuas xws li CV yuav tsum tau siv los sib piv tus cwj pwm hloov pauv.CV siab qhia qhov sib txawv loj ntawm kev kho mob rau qhov zoo.Cov txiaj ntsig ntawm qhov kev sim no tau pom qhov sib txawv loj ntawm kev kho cov suab thaj qis hauv cov hauv paus qhuav qhov hnyav, cov hauv paus tshiab, qhov sib piv ntawm carbohydrates-rau-qab zib, thiab cov yam ntxwv hauv paus.
ANOVA cov txiaj ntsig tau pom tias piv rau kev tswj hwm, foliar spraying nrog gibberellic acid thiab benzyladenine tau muaj txiaj ntsig zoo rau cov nroj tsuag qhov siab, tus naj npawb ntawm nplooj, thaj chaw nplooj, cov hauv paus ntim, lub hauv paus ntev, chlorophyll Performance index, qhov hnyav thiab qhov hnyav qhuav.
Kev sib piv ntawm cov txiaj ntsig tau pom tau tias cov kev tswj hwm kev loj hlob ntawm cov nroj tsuag muaj txiaj ntsig zoo rau cov nroj tsuag qhov siab thiab nplooj ntawv naj npawb.Cov kev kho mob zoo tshaj plaws yog gibberellic acid ntawm qhov concentration ntawm 200 mg / l thiab gibberellic acid + benzyladenine ntawm qhov concentration ntawm 200 mg / l.Piv nrog rau kev tswj hwm, cov nroj tsuag qhov siab thiab cov nplooj nce los ntawm 32.92 npaug thiab 62.76 zaug, raws li (Table 2).
Nplooj thaj tsam tau nce ntxiv hauv txhua qhov sib txawv piv rau kev tswj hwm, nrog qhov siab tshaj plaws tau pom ntawm 200 mg / l rau gibberellic acid, mus txog 89.19 cm2.Cov txiaj ntsig tau pom tias thaj chaw nplooj ntoo nce ntxiv nrog kev loj hlob regulator concentration (Table 2).
Tag nrho cov kev kho mob tseem ceeb nce hauv paus ntim thiab ntev piv rau kev tswj.Kev sib xyaw ua ke ntawm gibberellic acid + benzyladenine muaj cov txiaj ntsig zoo tshaj plaws, ua kom lub ntim thiab qhov ntev ntawm cov cag los ntawm ib nrab piv rau kev tswj hwm (Table 2).
Cov txiaj ntsig siab tshaj plaws ntawm lub qia txoj kab uas hla thiab ntev internode tau pom nyob rau hauv kev tswj hwm thiab gibberellic acid + benzyladenine 200 mg / l kev kho mob, feem.
Qhov ntsuas chlorophyll tau nce hauv txhua qhov sib txawv piv rau kev tswj hwm.Tus nqi siab tshaj ntawm qhov kev zoo no tau pom thaum kho nrog gibberellic acid + benzyladenine 200 mg / l, uas yog 30.21% siab dua li kev tswj hwm (Table 2).
Cov txiaj ntsig tau pom tias kev kho mob ua rau muaj qhov sib txawv ntawm cov xim pigment, txo cov suab thaj thiab soluble carbohydrates.
Kev kho mob nrog gibberellic acid + benzyladenine ua rau cov ntsiab lus siab tshaj plaws ntawm photosynthetic pigments.Qhov kos npe no tau nce siab dua hauv txhua qhov sib txawv dua li hauv kev tswj hwm.
Cov txiaj ntsig tau pom tias txhua qhov kev kho mob tuaj yeem ua rau cov ntsiab lus chlorophyll ntawm Schefflera dwarf.Txawm li cas los xij, tus nqi siab tshaj plaws ntawm qhov kev coj cwj pwm no tau pom nyob rau hauv kev kho mob nrog gibberellic acid + benzyladenine, uas yog 36.95% siab dua li kev tswj hwm (Table 3).
Cov txiaj ntsig rau chlorophyll b tau zoo ib yam li cov txiaj ntsig rau chlorophyll a, qhov sib txawv tsuas yog qhov nce ntawm cov ntsiab lus ntawm chlorophyll b, uas yog 67.15% siab dua hauv kev tswj hwm (Table 3).
Kev kho mob ua rau muaj kev nce ntxiv hauv tag nrho chlorophyll piv rau kev tswj hwm.Kev kho mob nrog gibberellic acid 200 mg / l + benzyladenine 100 mg / l coj mus rau qhov muaj txiaj ntsig zoo tshaj plaws, uas yog 50% siab dua li kev tswj hwm (Table 3).Raws li qhov tshwm sim, kev tswj hwm thiab kev kho mob nrog benzyladenine ntawm koob tshuaj 100 mg / l coj mus rau qhov siab tshaj plaws ntawm qhov zoo.Liriodendron tulipifera muaj tus nqi siab tshaj ntawm carotenoids (Table 3).
Cov txiaj ntsig tau pom tias thaum kho nrog gibberellic acid ntawm qhov concentration ntawm 200 mg / L, cov ntsiab lus ntawm chlorophyll nce ntxiv rau chlorophyll b (Fig. 1).
Cov nyhuv ntawm gibberellic acid thiab benzyladenine ntawm a / b Ch.Feem ntau ntawm dwarf schefflera.(GA3: gibberellic acid thiab BA: benzyladenine).Cov ntawv tib yam hauv txhua daim duab qhia tias qhov sib txawv tsis tseem ceeb (P <0.01).
Cov txiaj ntsig ntawm txhua qhov kev kho mob ntawm qhov hnyav thiab qhuav ntawm cov ntoo dwarf schefflera tau nce siab dua li kev tswj hwm.Gibberellic acid + benzyladenine ntawm 200 mg / L yog qhov kev kho mob zoo tshaj plaws, nce qhov hnyav ntawm 138.45% piv rau kev tswj hwm.Piv nrog rau kev tswj hwm, tag nrho cov kev kho mob tshwj tsis yog 100 mg / L benzyladenine ua rau cov nroj tsuag qhuav hnyav, thiab 200 mg / L gibberellic acid + benzyladenine ua rau muaj txiaj ntsig zoo tshaj plaws rau qhov zoo (Table 4).
Feem ntau ntawm cov kev hloov pauv txawv ntawm kev tswj hwm hauv qhov kev hwm no, nrog rau cov txiaj ntsig siab tshaj plaws ntawm 100 thiab 200 mg / l benzyladenine thiab 200 mg / l gibberellic acid + benzyladenine (Fig. 2).
Kev cuam tshuam ntawm gibberellic acid thiab benzyladenine ntawm qhov sib piv ntawm soluble carbohydrates thiab txo cov suab thaj hauv dwarf schefflera.(GA3: gibberellic acid thiab BA: benzyladenine).Cov ntawv tib yam hauv txhua daim duab qhia tias tsis muaj qhov sib txawv tseem ceeb (P <0.01).
Stepwise regression tsom xam tau ua los txiav txim siab tus cwj pwm tiag tiag thiab nkag siab zoo txog kev sib raug zoo ntawm tus kheej hloov pauv thiab nplooj ntawv tus lej hauv Liriodendron tulipifera.Hauv paus ntim yog thawj qhov sib txawv nkag mus rau hauv tus qauv, piav qhia 44% ntawm qhov sib txawv.Qhov sib txawv tom ntej no yog qhov hnyav hauv paus, thiab ob qhov sib txawv no tau piav qhia 63% ntawm qhov sib txawv ntawm nplooj ntawv (Table 5).
Txoj kev tsom xam tau ua kom zoo dua txhais cov kauj ruam rov qab (Table 6 thiab Daim duab 3).Qhov txiaj ntsig zoo tshaj plaws ntawm cov nplooj ntawv tau cuam tshuam nrog cov hauv paus tshiab (0.43), uas muaj kev cuam tshuam zoo nrog nplooj ntawv (0.47).Qhov no qhia tau hais tias qhov kev coj cwj pwm no cuam tshuam ncaj qha rau cov txiaj ntsig, thaum nws cov txiaj ntsig tsis ncaj ncees los ntawm lwm yam tsis zoo, thiab qhov kev coj cwj pwm no tuaj yeem siv los ua cov txheej txheem xaiv hauv kev yug me nyuam rau dwarf schefflera.Qhov cuam tshuam ncaj qha ntawm lub hauv paus ntim tsis zoo (−0.67).Kev cuam tshuam ntawm qhov zoo ntawm cov nplooj ntawm cov nplooj yog ncaj qha, qhov cuam tshuam tsis ncaj ncees yog qhov tsis tseem ceeb.Qhov no qhia tau hais tias qhov loj ntawm lub hauv paus ntim, qhov me me ntawm cov nplooj.
Daim duab 4 qhia txog kev hloov pauv hauv cov kab rov tav ntawm cov hauv paus ntim thiab txo cov suab thaj.Raws li cov regression coefficient, txhua chav hloov pauv hauv paus ntev thiab soluble carbohydrates txhais tau hais tias hauv paus ntim thiab txo cov suab thaj hloov los ntawm 0.6019 thiab 0.311 units.
Pearson correlation coefficient ntawm kev loj hlob zoo yog qhia nyob rau hauv daim duab 5. Cov txiaj ntsig tau pom tias cov nplooj thiab cov nroj tsuag qhov siab (0.379*) muaj qhov sib raug zoo thiab qhov tseem ceeb tshaj plaws.
Daim ntawv qhia tshav kub ntawm kev sib raug zoo ntawm qhov sib txawv hauv kev loj hlob tus nqi correlation coefficients.# Y Axis: 1-Index Ch., 2-Internode, 3-LAI, 4-N ntawm nplooj, 5-Qhov siab ntawm ceg, 6-kab kab.# Raws li X axis: A – Performance index H., B – nrug ntawm nodes, C – LAY, D – N. ntawm nplooj, E – qhov siab ntawm lub ris ceg, F – txoj kab uas hla ntawm qia.
Pearson correlation coefficient rau ntub qhov hnyav-txog yam ntxwv muaj nyob rau hauv daim duab 6. Cov txiaj ntsig tau qhia txog kev sib raug zoo ntawm cov nplooj ntub dej thiab qhov hnyav saum av qhuav (0.834**), tag nrho qhov hnyav qhuav (0.913**) thiab lub hauv paus qhuav qhov hnyav (0.562*. )..Tag nrho cov huab hwm coj qhuav muaj qhov zoo tshaj plaws thiab tseem ceeb tshaj plaws kev sib raug zoo nrog tua qhuav huab cua (0.790**) thiab hauv paus qhuav huab cua (0.741**).
Thaum tshav kub kub daim ntawv qhia ntawm kev sib raug zoo ntawm tshiab hnyav correlation coefficient variables.# Y axis: 1 - qhov hnyav ntawm nplooj tshiab, 2 - qhov hnyav ntawm cov nplooj tshiab, 3 - qhov hnyav ntawm cov hauv paus hniav tshiab, 4 - tag nrho qhov hnyav ntawm nplooj tshiab.# X-axis sawv cev: A - cov nplooj tshiab hnyav, B - tshiab bud hnyav, CW - cov cag tshiab, D - tag nrho qhov hnyav tshiab.
Pearson correlation coefficients rau qhuav qhov hnyav-txog yam ntxwv muaj nyob rau hauv daim duab 7. Cov txiaj ntsig tau qhia tias nplooj qhuav qhov hnyav, bud qhuav hnyav (0.848**) thiab tag nrho qhov hnyav qhuav (0.947**), bud qhuav hnyav (0.854**) thiab tag nrho cov huab hwm coj qhuav (0.781**) muaj qhov tseem ceeb tshaj plaws.zoo correlation thiab tseem ceeb correlation.
Thaum tshav kub kub daim ntawv qhia kev sib raug zoo ntawm qhuav hnyav correlation coefficient variables.# Y axis sawv cev: 1-nplooj qhuav hnyav, 2-pob qhuav hnyav, 3-hauv paus qhuav hnyav, 4-tag nrho qhov hnyav.# X Axis: A-nplooj qhuav qhov hnyav, B-bud qhuav hnyav, CW cag qhuav hnyav, D-tag nrho qhov hnyav qhuav.
Pearson correlation coefficient ntawm cov xim pigment yog qhia hauv daim duab 8. Cov txiaj ntsig tau pom tias chlorophyll a thiab chlorophyll b (0.716**), tag nrho chlorophyll (0.968**) thiab tag nrho cov pigments (0.954**);chlorophyll b thiab tag nrho chlorophyll (0.868**) thiab tag nrho cov xim (0.851**);tag nrho chlorophyll muaj qhov zoo tshaj plaws thiab kev sib raug zoo nrog tag nrho cov xim (0.984**).
Daim ntawv qhia tshav kub ntawm kev sib raug zoo ntawm chlorophyll correlation coefficient variables.# Y axes: 1- Channel a, 2- Channel.b, 3 – a/b ratio, 4 channels.Tag nrho, 5-carotenoids, 6-yield pigments.# X-Axes: A-Ch.aB-Ch.b, C-a/b ratio, D-Ch.Tag nrho cov ntsiab lus, E-carotenoids, F-yield ntawm pigments.
Dwarf Schefflera yog ib tsob ntoo nrov thoob plaws ntiaj teb, thiab nws txoj kev loj hlob thiab kev loj hlob tam sim no tau txais kev saib xyuas ntau.Kev siv cov tswj kev loj hlob ntawm cov nroj tsuag ua rau muaj qhov sib txawv loj, nrog rau txhua qhov kev kho mob nce cog qhov siab piv rau kev tswj.Txawm hais tias cov nroj tsuag qhov siab feem ntau yog tswj cov caj ces, kev tshawb fawb qhia tau hais tias daim ntawv thov ntawm cov nroj tsuag kev loj hlob regulators tuaj yeem nce lossis txo cov nroj tsuag qhov siab.Cov nroj tsuag qhov siab thiab pes tsawg nplooj kho nrog gibberellic acid + benzyladenine 200 mg / L yog qhov siab tshaj plaws, ncav cuag 109 cm thiab 38.25, raws li.Raws li kev tshawb fawb yav dhau los (SalehiSardoei et al.52) thiab Spathiphyllum23, zoo sib xws hauv cov nroj tsuag qhov siab vim gibberellic acid kho tau pom nyob rau hauv potted marigolds, albus alba21, daylilies22, daylilies, agarwood thiab kev thaj yeeb lilies.
Gibberellic acid (GA) plays lub luag haujlwm tseem ceeb hauv ntau yam txheej txheem physiological ntawm cov nroj tsuag.Lawv txhawb nqa cell faib, cell elongation, qia elongation thiab loj hlob 24.GA induces cell faib thiab elongation nyob rau hauv tua apices thiab meristems25.Nplooj hloov kuj suav nrog txo qis qia tuab, nplooj me me, thiab xim ntsuab dua 26.Cov kev tshawb fawb siv inhibitory lossis stimulatory yam tau pom tias calcium ions los ntawm cov chaw sab hauv ua raws li cov tub txib thib ob hauv gibberellin signaling txoj hauv kev sorghum corolla27.HA nce cog ntev los ntawm kev txhawb nqa cov synthesis ntawm cov enzymes uas ua rau cov phab ntsa ntawm tes so, xws li XET lossis XTH, expansins thiab PME28.Qhov no ua rau cov hlwb kom loj thaum lub cell phab ntsa so thiab dej nkag mus rau hauv cell29.Daim ntawv thov ntawm GA7, GA3 thiab GA4 tuaj yeem ua kom cov qia elongation30,31.Gibberellic acid ua rau qia elongation nyob rau hauv dwarf nroj tsuag, thiab nyob rau hauv rosette nroj tsuag nws retards nplooj loj hlob thiab internode elongation32.Txawm li cas los xij, ua ntej lub sijhawm yug me nyuam, lub qia ntev nce mus rau 4-5 npaug ntawm nws qhov siab qub 33.Cov txheej txheem ntawm GA biosynthesis hauv cov nroj tsuag tau sau tseg hauv daim duab 9.
GA biosynthesis hauv cov nroj tsuag thiab theem ntawm endogenous bioactive GA, schematic sawv cev ntawm cov nroj tsuag (txoj cai) thiab GA biosynthesis (sab laug).Cov xub yog xim coded kom sib haum rau daim ntawv HA qhia raws txoj kev biosynthetic;Cov xub liab qhia tias txo GC qib vim qhov chaw nyob hauv cov kab mob hauv nruab nrog cev, thiab cov xub dub qhia tias nce GC qib.Hauv ntau cov nroj tsuag, xws li mov thiab txiv tsawb, GA cov ntsiab lus siab dua ntawm lub hauv paus lossis qis dua ntawm nplooj 30.Ntxiv mus, qee cov ntaub ntawv qhia tias bioactive GA cov ntsiab lus txo qis raws li nplooj elongate los ntawm lub hauv paus34.Cov theem tseeb ntawm gibberellins hauv cov xwm txheej no tsis paub.
Cog kev loj hlob regulators kuj tseem cuam tshuam rau tus naj npawb thiab cheeb tsam ntawm nplooj.Cov txiaj ntsig tau pom tias nce qhov concentration ntawm cov nroj tsuag kev loj hlob regulator ua rau muaj kev nce ntxiv ntawm nplooj nplooj thiab tus lej.Benzyladenine tau tshaj tawm tias yuav nce calla nplooj ntau lawm15.Raws li cov txiaj ntsig ntawm txoj kev tshawb no, tag nrho cov kev kho mob txhim kho nplooj nplooj thiab tus lej.Gibberellic acid + benzyladenine yog qhov kev kho mob zoo tshaj plaws thiab ua rau muaj ntau tshaj plaws thiab thaj chaw ntawm nplooj.Thaum loj hlob dwarf schefflera nyob hauv tsev, tej zaum yuav muaj qhov pom ntawm cov nplooj.
GA3 kev kho mob nce internode ntev piv nrog benzyladenine (BA) lossis tsis muaj tshuaj hormonal.Qhov txiaj ntsig no yog qhov laj thawj muab lub luag haujlwm ntawm GA hauv kev txhawb nqa kev loj hlob 7.Kev loj hlob ntawm qia kuj pom tau zoo sib xws.Gibberellic acid nce qhov ntev ntawm qia tab sis txo nws txoj kab uas hla.Txawm li cas los xij, kev sib xyaw ua ke ntawm BA thiab GA3 tau nce qia ntev.Qhov no nce siab dua piv rau cov nroj tsuag kho nrog BA lossis tsis muaj cov tshuaj hormones.Txawm hais tias gibberellic acid thiab cytokinins (CK) feem ntau txhawb kev loj hlob ntawm cov nroj tsuag, qee zaum lawv muaj qhov cuam tshuam rau cov txheej txheem sib txawv35.Piv txwv li, qhov kev cuam tshuam tsis zoo tau pom nyob rau hauv qhov nce hauv hypocotyl ntev hauv cov nroj tsuag kho nrog GA thiab BA36.Ntawm qhov tod tes, BA nce hauv paus ntim ntau heev (Table 1).Kev nce hauv paus ntim vim exogenous BA tau tshaj tawm hauv ntau cov nroj tsuag (xws li Dendrobium thiab Orchid hom) 37,38.
Txhua txoj kev kho hormonal nce cov nplooj tshiab.Ntuj nce nyob rau hauv nplooj nplooj thiab qia ntev los ntawm kev kho mob ua ke yog qhov xav tau kev lag luam.Tus naj npawb ntawm nplooj tshiab yog ib qho tseem ceeb qhia txog kev loj hlob vegetative.Kev siv cov tshuaj hormones exogenous tsis tau siv rau hauv kev lag luam ntau lawm ntawm Liriodendron tulipifera.Txawm li cas los xij, kev txhawb nqa kev loj hlob ntawm GA thiab CK, siv rau hauv kev sib npaug, tuaj yeem muab kev nkag siab tshiab rau kev txhim kho kev cog qoob loo ntawm cov nroj tsuag no.Qhov tseem ceeb, cov nyhuv synergistic ntawm BA + GA3 kev kho mob siab dua li ntawm GA lossis BA tswj hwm ib leeg.Gibberellic acid nce cov nplooj tshiab.Raws li cov nplooj tshiab tsim, nce cov nplooj tshiab tuaj yeem txwv cov nplooj loj hlob39.GA tau tshaj tawm los txhim kho kev thauj khoom ntawm sucrose los ntawm dab dej mus rau hauv lub cev 40,41.Tsis tas li ntawd, exogenous daim ntawv thov ntawm GA rau cov nroj tsuag perennial tuaj yeem txhawb txoj kev loj hlob ntawm cov kab mob xws li nplooj thiab cov hauv paus hniav, yog li tiv thaiv kev hloov pauv ntawm cov nroj tsuag mus rau kev loj hlob ntawm kev loj hlob 42.
Cov nyhuv ntawm GA ntawm cov nroj tsuag qhuav tuaj yeem piav qhia los ntawm kev nce hauv photosynthesis vim qhov nce ntawm nplooj nplooj43.GA tau tshaj tawm tias ua rau muaj qhov nce ntawm nplooj nplooj ntawm Maize34.Cov txiaj ntsig tau pom tias nce BA concentration mus rau 200 mg / L tuaj yeem ua kom qhov ntev thiab tus naj npawb ntawm cov ceg txuas ntxiv thiab cov hauv paus ntim.Gibberellic acid cuam tshuam cov txheej txheem ntawm tes xws li txhawb nqa cell faib thiab elongation, yog li txhim kho kev loj hlob 43.Tsis tas li ntawd, HA nthuav cov phab ntsa ntawm tes los ntawm hydrolyzing starch rau hauv qab zib, yog li txo cov dej hauv cell, ua rau cov dej nkag mus rau hauv lub cell thiab thaum kawg ua rau cell elongation44.

 


Post lub sij hawm: Jun-11-2024